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JoVE Journal Neuroscience
Laterale Diffusion und Exozytose von Membranproteinen in kultivierten Neuronen Veranlagte mit Fluorescence Recovery-und Fluoreszenz-Verlust Photobleaching
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Laterale Diffusion und Exozytose von Membranproteinen in kultivierten Neuronen Veranlagte mit Fluorescence Recovery-und Fluoreszenz-Verlust Photobleaching

Article DOI: 10.3791/3747 • 11:59 min • February 29th, 2012
February 29th, 2012 •
1, 1, 1, 1
1MRC Centre for Synaptic Plasticity, University of Bristol

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Dieser Bericht beschreibt die Verwendung von Live Cell Imaging und Photobleichmittels Techniken, um die Oberfläche Ausdruck, Transportwege und-handel Kinetik der exogen exprimiert, pH-sensitiven GFP-markierten Proteine ​​an der Plasmamembran von Nervenzellen zu ermitteln.

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Neuroscience Ausgabe 60 Fluorescence Recovery After Photobleaching FRAP konfokale Bildgebung Fluorophor GFP Super-Ekliptik im Gegensatz zu GFP September Fluoreszenz-Verlust in Photobleichmittels FLIP Neuron Protein-Verkehr Synapse
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