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Biology

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Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
 

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases

Article DOI: 10.3791/3749
December 26th, 2011

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Summary December 26th, 2011

Using specific glycosidases to remove sugars from glycoproteins followed by SDS-PAGE is a valuable method to detect glycan modifications on protein samples and is a good choice for initial glycobiology studies. Changes following deglycosylation can be detected as shifts in gel mobility or by staining with glycan sensitive reagents.

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