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JoVE Journal
Immunology and Infection

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Colonization of Euprymna scolopes Squid by Vibrio fischeri
 

Colonization of Euprymna scolopes Squid by Vibrio fischeri

Article DOI: 10.3791/3758
March 1st, 2012

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Summary March 1st, 2012

The method outlines the procedure by which the Hawaiian bobtail squid, Euprymna scolopes and its bacterial symbiont, Vibrio fischeri, are raised separately and then introduced to allow for specific colonization of the squid light organ by the bacteria. Colonization detection by bacterially-derived luminescence and by direct colony counting are described.

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