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Medicine
A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue...
A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue...
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JoVE Journal Medicine
A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe

A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe

Full Text
21,963 Views
14:58 min
June 12, 2012

DOI: 10.3791/3765-v

Mohit Parekh1, Stefano Ferrari1, Enzo Di Iorio1, Vanessa Barbaro1, Davide Camposampiero1, Marianthi Karali2, Diego Ponzin1, Gianni Salvalaio1

1Fondazione Banca Degli Occhi del Veneto O.N.L.U.S. , 2Telethon Institute for Genetics & Medicine (T.I.G.E.M.)

The paper describes a simplified technique to excise corneal and to eviscerate retinal tissues from the ocular globe of human cadaveric donors. The technique described here will help to excise good quality tissues to be used for transplantation, surgical or research purposes without damaging other tissues of the ocular globe.

Today We are demonstrating a simplified technique to exercise corneal and to ate retinal tissue from a human ocular globe of cadaveric donors. The technique described the year will help to exercise good quality tissue to be used for transplantation, surgical, oral research purposes without damaging other tissue of the ocular globe. With 18 years of expertise and Andres of cornea transplanted each year, we have maintained a good standard of our work by developing new techniques and improving our skill every day.

To achieve this, we exercise the cornea first, followed by retinal evis. The cornea is an avascular tissue which enables the transmission of light onto the retina, and for this purpose, should always maintain a good degree of transparency. The cornea comprises five layers, the epithelium, bowmans, layer, stroma, desus membrane, and endothelium.

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