Aseptic Technique
Aseptic Technique - Related Videos
10:48
Aseptic Technique in Environmental Science
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner Aseptic technique is a fundamental skill widely practiced in the field of environmental microbiology that requires a balance of mindfulness and practice in the laboratory. Proper use of this technique reduces the likelihood of bacterial or fungal contamination of reagents, culture media, and environmental samples. Aseptic technique is also vital to ensure data integrity and...
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
09:46
Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening
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Cited by 9 •
2018
This manuscript describes a murine calvarial osteolysis model by exposure to CoCrMo particles, which constitutes an ideal animal model for assessing the interactions between wear particles and various cells in aseptic loosening.
12:40
A Modified Murine Heterotopic Heart Transplant Protocol Matching Contemporary Standards of Aseptic Technique, Anesthesia, and Analgesia
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2022
The present paper describes a modified technique for heterotopic vascularized cardiac transplantation with updated aseptic technique, analgesia, and anesthesia.
FAQs
Unwanted microorganisms can be transferred through air, surfaces, personnel, or contact with nonsterile items. Aseptic technique addresses these routes by combining hand hygiene, surface disinfection, sterilized instruments, sterile containers, and controlled handling. Managing several possible transfer pathways is important because contamination may otherwise enter cultures or reagents without being part of the intended experiment.
Each practice limits contamination at a different point in the workflow. Hand hygiene reduces transfer by personnel, surface disinfection addresses contaminated work areas, and sterilized instruments reduce the chance that tools introduce microorganisms. Sterile containers and controlled handling provide additional protection for materials after preparation, so no single measure carries the entire burden of contamination control.
Consistent application helps keep unwanted microbial growth from becoming a variable between experiments. If contamination alters a culture, tissue sample, or reagent, observed changes may reflect microorganisms rather than the biological process under study. Applying the same protective practices across comparable procedures therefore supports reliable results and makes differences easier to interpret as true biological effects.
A basic workflow combines hand hygiene, preparation of disinfected surfaces, use of sterilized instruments and sterile containers, and controlled handling of materials. These practices should be maintained while working with cultures, tissues, reagents, or other sterile items. The workflow is not a single action; it is a coordinated sequence that limits opportunities for contact with contaminants.
Sterile materials, cultures, experimental environments, reagents, instruments, and containers all require controlled handling. Surfaces must be disinfected, instruments must be sterilized, and personnel must practice hand hygiene before and during work. Attention to each component helps preserve the condition of biological materials and reduces the chance that contamination will be introduced during preparation or transfer.
Researchers apply it during cell culture, microbial culture, tissue handling, and reagent preparation. These activities involve materials whose results can be affected by unintended microbial growth, making contamination control especially important. Using aseptic practices helps preserve cultures and samples while allowing researchers to evaluate the intended biological system rather than changes caused by introduced microorganisms.
By limiting unintended microbial growth, aseptic technique helps separate the effects of the planned biological treatment or process from contamination-related changes. This distinction is important when evaluating cultures, tissues, or reagents. Cleaner handling supports reproducibility across experiments and increases confidence that differences in biological results reflect the system being investigated rather than inconsistent microbial transfer.