Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

The overall goal of this procedure is to induce EAE consistently and easily in any mouse strain, including those reputed to be resistant to EAE induction. This is accomplished by first immunizing donor mice with myelin specific proteins or peptide antigens, emulsified in complete fres adjuvant. 10 days later, the lymph nodes from the donor mice are isolated and lymph node cells are re stimulated in vitro by culturing them with the same antigen for four to five days.

The cells are then adoptively transferred into naive recipient mice. Susceptible mouse strains will develop EAE in eight to 14 days to trigger EAE in a resistant strain. 14 days after the adoptive transfer, the recipient mice were then immunized with antigen in CFA.