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Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
DOI:

13:28 min

February 15, 2012

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Chapters

  • 00:05Title
  • 01:42Labeling B. subtilis and Biofilm Formation Assay
  • 05:30Biofilm Dispersion and Cell Fixation
  • 06:28Fluorescence Microscopy
  • 07:22Quantification of Single Cells Using Flow Cytometry
  • 08:47Results of B. subtilis Biofilm Analysis
  • 13:01Conclusion

Summary

Automatic Translation

Microbial biofilms are generally constituted by distinct subpopulations of specialized cells. Single-cell analysis of these subpopulations requires the use of fluorescent reporters. Here we describe a protocol to visualize and monitor several subpopulationswithin B. subtilis biofilms using fluorescence microscopy and flow cytometry.

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