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Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
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Chapters
- 00:05Title
- 01:22Equipment and Electrode Preparation
- 04:05Preparation of Dopamine Neuronal Culture and Patch-clamp Recording
- 06:12Results: Whole-cell and Amperometric Recording at Multiple Voltages
- 06:46Conclusion
The amperometric technique measures dopamine release from a single cell by detecting the oxidative current produced by spontaneous dopamine oxidization. Simultaneous voltage clamp and amperometry methodology reveal the mechanistic relationship between the overall "activity" of dopamine transporter and the regulatory role of this activity on the reverse transport of dopamine.
Tags
Single Cell MeasurementDopamine ReleaseVoltage-clampAmperometrySynaptic CleftPre-synaptic TargetsPost-synaptic TargetsDiffusionExtracellular EnzymesMembrane TransportersDopamine TransporterPeri-synaptic CleftInward Dopamine FluxUptakeOutward TransportEfflux Of DopamineSulzer Et Al.Forward ActivityReverse ActivityExtracellular Dopamine HomeostasisPatch Clamp RecordingAmperometric RecordingMillisecond Time ResolutionMembrane Potential Control
