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November 21, 2012
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The amperometric technique measures dopamine release from a single cell by detecting the oxidative current produced by spontaneous dopamine oxidization. Simultaneous voltage clamp and amperometry methodology reveal the mechanistic relationship between the overall "activity" of dopamine transporter and the regulatory role of this activity on the reverse transport of dopamine.
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Cite this Article
Saha, K., Swant, J., Khoshbouei, H. Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry. J. Vis. Exp. (69), e3798, doi:10.3791/3798 (2012).
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