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Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
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Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

DOI:
10.3791/3804-v

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08:25 min

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April 03, 2012

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, ,
1Yale Stem Cell Center, Department of Genetics,Yale School of Medicine

Chapters

  • 00:05Title
  • 01:28Infecting Cells with Retrovirus Expression Reprogramming Factors
  • 03:31Isolation and Expansion of Induced Pluripotent Stem Cells
  • 04:42Analysis of Pluripotent Markers by Immunofluorescence and Quantitative Real-time PCR
  • 06:06Representative Cell-reprogramming Results
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.

Tags

ReprogrammingHuman Somatic CellsInduced Pluripotent Stem CellsRetroviral VectorGFPHuman Embryonic Stem CellsPluripotentIn Vitro Disease ModelingRegenerative MedicineTranscription FactorsOct4Sox2Klf4MycAutologous CellsFibroblast CellsGFP-containing Retroviral BackboneESC Culture ConditionMorphologyRetroviral Transgene SilencingFluorescence MicroscopePluripotency GenesSurface Markers

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