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Chapters
- 00:05Title
- 01:50The Concept of FRET
- 03:16Synthesis of PDA
- 05:52Liposome Preparation
- 07:46Preparation of Rhodamine-tagged Bovine Serum Albumin (BSA-Rh) Modified PCDA Liposomes
- 09:38Representative FRET Results
- 11:45Conclusion
We demonstrate FRET between conjugated polymer polydiacetylene (PDA) and fluorophore attached to the surface of PDA liposomes for the sensing of biomolecules. PDA liposomes also contained receptor molecules on their surfaces for biomolecules to be used as probes. Ligand-receptor interactions lead to changes in the FRET efficiency between the fluorophore and PDA which is the basis of the sensing mechanism.
Tags
Real-time MonitoringLigand-receptor InteractionsFluorescence Resonance Energy TransferFRETPDAUV-Vis Electronic Absorption SpectrumAnalyteReceptorsSpectral OverlapJRhodamineFRET EfficiencyDonor FluorophoresModel Protein Molecule StreptavidinCovalent-bindingBovine Serum AlbuminLiposome SurfaceBilayer LiposomesReal-time SensingSmall Chemical MoleculesLarge Biochemical MoleculesDetection Limit
