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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Article DOI: 10.3791/3805
August 20th, 2012 •
1, 1, 1, 1
1Department of Chemistry and Biochemistry, Southern Illinois University

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August 20th, 2012

We demonstrate FRET between conjugated polymer polydiacetylene (PDA) and fluorophore attached to the surface of PDA liposomes for the sensing of biomolecules. PDA liposomes also contained receptor molecules on their surfaces for biomolecules to be used as probes. Ligand-receptor interactions lead to changes in the FRET efficiency between the fluorophore and PDA which is the basis of the sensing mechanism.

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