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Isolate A Genetik Ekran Toxoplasma gondii Host-hücre çıkış Mutants
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Chapters
- 00:05Title
- 01:39Titration of Mutagen
- 05:59Enrichment of Egress Mutants
- 08:48Validation of Mutant Phenotypes by Egress Assays
- 10:21Cloning of Egress Mutants by Limiting Dilution
- 12:41Representative Data
- 14:14Conclusion
İleri genetik, moleküler düzeyde nasıl aydınlatmak için güçlü bir yöntemdir<em> Toxoplasma</em> Onun konakçı hücre gelen egresses. Protokoller, kimyasal parazitler mutagenize için sağlanan indüklenen çıkış defekti olan mutantlar için zenginleştirmek, ve klonlanmış mutantlar fenotip doğrulamak.
Tags
Genetic ScreenToxoplasma GondiiHost-cell Egress MutantsObligate Intracellular ParasiteLytic Replication CycleActive InvasionReplication Inside Host CellActive EgressMolecular LevelSignaling PathwaysPharmacological AgentsTriggers Of EgressIntracellular Ca2+Candidate Gene ApproachPlant Like Calcium Dependent Protein Kinase (CDPK)(chemical) Genetic ApproachesScreen For Mutant ParasitesDefect In Host Cell EgressChemical MutagenesisN-ethyl-N-nitrosourea (ENU)
