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Single-cell Profiling of Developing and Mature Retinal Neurons
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Neuroscience
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JoVE Journal Neuroscience
Single-cell Profiling of Developing and Mature Retinal Neurons

Single-cell Profiling of Developing and Mature Retinal Neurons

DOI:
10.3791/3824-v

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10:20 min

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April 19, 2012

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,
1Department of Genetics, Development and Cell Biology, Neuroscience Program,Iowa State University

Chapters

  • 00:05Title
  • 02:07Cell Dissociation
  • 03:44Harvesting Single Cells
  • 05:15Reverse Transcription
  • 06:45Tailing and Single-cell PCR
  • 07:15Labeling for Affymetrix Chips
  • 08:23Results: Single-cell Profiling of Developing and Mature Retinal Neurons
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.

Tags

Single-cell ProfilingDeveloping Retinal NeuronsMature Retinal NeuronsCell-type Specific MarkersGene Expression ProgramsIndividual CellsTransient Gene Expression ProgramsCellular DiversityCentral Nervous SystemDistinct Cell TypesComplex Neural CircuitsRare Neuronal TypesMolecular ClassificationAnalysis Of Cell TypesNervous SystemMechanisms Of Neural Development

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