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JoVE Journal
Neuroscience

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Single-cell Profiling of Developing and Mature Retinal Neurons
 

Single-cell Profiling of Developing and Mature Retinal Neurons

Article DOI: 10.3791/3824
April 19th, 2012

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Summary April 19th, 2012

A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.

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