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Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
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Chapters
- 00:05Title
- 01:33Build the Storage Housing for the Microspheres
- 03:00Populating the Surface with Hydroxyl Groups
- 03:52Attaching Silane Coupling Agents to the Surface
- 04:42Attaching Biotin to the Surface
- 06:35Fluorescent Labeling of Biotin-terminated Silica
- 07:57Results: Functionalized Microspheres as Identified Through Optical and Fluorescence Microscopy
- 08:57Conclusion
Biosensors interface with complex, biological environments and perform targeted detection by combining highly sensitive sensors with highly specific probes attached to the sensor via surface modification. Here, we demonstrate the surface functionalization of silica optical sensors with biotin using silane coupling agents to bridge the sensor and the biological environment.
Tags
Biological ProbesSilica Optical BiosensorsSilane Coupling AgentsSurface Modification TechniquesHydrophobicityHydrophilicityElectronic EnvironmentsSpecificityLabel-free Biosensing PlatformsWhispering Gallery Mode (WGM) Optical ResonatorsUltra-low ConcentrationsGeometric OpticsResonance FrequenciesEvanescent Tail
