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Colocar Sondas Biológicas para Biossensores ópticos de sílica usando agentes de acoplamento de silano
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Chapters
- 00:05Title
- 01:33Build the Storage Housing for the Microspheres
- 03:00Populating the Surface with Hydroxyl Groups
- 03:52Attaching Silane Coupling Agents to the Surface
- 04:42Attaching Biotin to the Surface
- 06:35Fluorescent Labeling of Biotin-terminated Silica
- 07:57Results: Functionalized Microspheres as Identified Through Optical and Fluorescence Microscopy
- 08:57Conclusion
Biossensores interface com ambientes complexos e biológicas e realizar a detecção de alvo, combinando sensores altamente sensíveis com sondas altamente específicos ligados ao sensor através de modificação da superfície. Aqui, nós demonstrar a funcionalização da superfície de sensores ópticos de sílica com biotina, utilizando agentes de acoplamento de silano para colmatar o sensor eo ambiente biológico.
Tags
Biological ProbesSilica Optical BiosensorsSilane Coupling AgentsSurface Modification TechniquesHydrophobicityHydrophilicityElectronic EnvironmentsSpecificityLabel-free Biosensing PlatformsWhispering Gallery Mode (WGM) Optical ResonatorsUltra-low ConcentrationsGeometric OpticsResonance FrequenciesEvanescent Tail
