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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

Article DOI: 10.3791/3874-v • 08:30 min • May 16th, 2012
May 16th, 2012 •
1, 2, 3, 1, 1,3,4
1Department of Laboratory Medicine & Pathobiology, University of Toronto, 2Division of Urology, Sunnybrook Health Sciences Centre, Toronto, Canada, 3Department of Anatomic Pathology, Sunnybrook Health Sciences Centre, Toronto, Canada, 4Biological Sciences, Sunnybrook Research Institute

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Summary
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Quantitative Real Time polymerase chain reaction (qPCR) is a rapid and sensitive method to investigate the expression levels of various microRNA (miRNA) molecules in tumor samples. Using this method expression of hundreds of different miRNA molecules can be amplified, quantified, and analyzed from the same cDNA template.

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MicroRNA Detection Prostate Tumors Quantitative Real-time PCR (qPCR) MiRNAs Non-coding RNA Molecules Cellular Process Development Apoptosis Cell Cycle Regulation Gene Expression Regulation Target Messenger RNAs (mRNAs) Dysregulation Biomarkers Therapeutic Agents Taqman Probe-based QPCR Clinical Samples Accuracy Reproducibility Specificity Whole Genome Analyses Microarray Studies Radical Prostatectomy Clinical Variables
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