Journal
/
Medicine
/
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

DOI:
10.3791/3874-v

•

08:30 min

•

May 16, 2012

•

, , , ,
1Department of Laboratory Medicine & Pathobiology,University of Toronto, 2Division of Urology,Sunnybrook Health Sciences Centre, Toronto, Canada, 3Department of Anatomic Pathology,Sunnybrook Health Sciences Centre, Toronto, Canada, 4Biological Sciences,Sunnybrook Research Institute

Chapters

  • 00:05Title
  • 01:51Prostate Sample Collection
  • 02:40Isolating Total RNA from Samples
  • 04:27Real-time PCR for Detection of miRNA
  • 06:10Analyzing the Data
  • 07:25Representative qPCR Results
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

Quantitative Real Time polymerase chain reaction (qPCR) is a rapid and sensitive method to investigate the expression levels of various microRNA (miRNA) molecules in tumor samples. Using this method expression of hundreds of different miRNA molecules can be amplified, quantified, and analyzed from the same cDNA template.

Tags

MicroRNA DetectionProstate TumorsQuantitative Real-time PCR (qPCR)MiRNAsNon-coding RNA MoleculesCellular ProcessDevelopmentApoptosisCell Cycle RegulationGene Expression RegulationTarget Messenger RNAs (mRNAs)DysregulationBiomarkersTherapeutic AgentsTaqman Probe-based QPCRClinical SamplesAccuracyReproducibilitySpecificityWhole Genome AnalysesMicroarray StudiesRadical ProstatectomyClinical Variables

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3874?article_type=v

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
check_url/3874?article_type=v

A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells
check_url/3874?article_type=v

Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging
check_url/3874?article_type=v

Detection and Genogrouping of Noroviruses from Children’s Stools By Taqman One-step RT-PCR
check_url/3874?article_type=v

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence
check_url/3874?article_type=v

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
check_url/3874?article_type=v

Movement Retraining using Real-time Feedback of Performance
check_url/3874?article_type=v

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
check_url/3874?article_type=v

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
check_url/3874?article_type=v

Real-time Cytotoxicity Assays in Human Whole Blood

Read Article