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Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
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Chapters
- 00:05Title
- 01:31Chip Fabrication and Sample Pre-treatment
- 03:42Serum Fractionation
- 05:27MALDI-TOF Analysis of Peptides
- 06:40Data Analysis
- 08:36Results: iManipulation of MSP Thin Films for Selective Protein Enrichment
- 12:24Conclusion
We developed a technology based on mesoporous silica thin film for the selective recovery of low molecular weight proteins and peptides from human serum. The physico-chemical properties of our mesoporous chips were finely tuned to provide substantial control in peptide enrichment and consequently profile the serum proteome for diagnostic purposes.
Tags
Low Molecular Weight Protein EnrichmentMesoporous Silica Thin FilmsBiomarker DiscoveryCirculating BiomarkersEarly DiagnosisPrognosisTherapeutic EfficiencyLow Molecular Weight Proteome (LMWP)Mass Spectrometry (MS)Dynamic RangeProtein And Peptide ConcentrationsSample Pre-treatmentHighly Abundant ProteinsLow-abundance SpeciesProteomic-based ApproachesTwo-dimensional Polyacrylamide Gel-electrophoresis (2D-PAGE)Shotgun Proteomics MethodsClinical ApplicationsIsolation Of LMWP From BloodHigh Throughput ScreeningMulti-fractionation SystemMesoporous Silica Chips
