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Chapters
- 00:05Title
- 01:44Pretreatment of Cells and Metaphase Preparation
- 05:16Pepsin Pretreatment of Slides
- 07:32Chromosome and Probe Denaturation and Hybridization
- 09:00Fluorescent Probe Design
- 10:53Representative Results from Spectral Karyotyping of Mouse and Human Chromosomes
- 12:31Conclusion
Spectral karyotyping (SKY) er en avanceret cytogenetik teknik til at identificere genomiske og kromosomforandringer. Denne teknik drager fordel af kromosom maleri prober, hvilket muliggøre klassificering af alle kromosomer. SKY kan også identificere komplekse kromosomafvigelser og adskillelse mangler i mus og mennesker med forskellige sygdomme, herunder polycystisk nyresygdom.
Tags
Spectral KaryotypingChromosome AbnormalitiesHumansMicePolycystic Kidney DiseaseBanding PatternClassical Banding TechniqueReliable IdentificationComplex Chromosomal AberrationsCancerSpectral Karyotyping (SKY)Multicolor Fluorescence In-situ Hybridization (FISH)Spectral MicroscopeCytogenetic AnalysisGenetic DiseasesMalignanciesMulticolor Fluorescently-labelled DNA ProbesDegenerate Oligonucleotide PrimersPCRUnique Spectral ColorIn-situ HybridizationFluorescent DyesRhodamineTexas RedCy5FITCCy5.5Chromosome Specific Probes
