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Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice
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JoVE Journal Neuroscience
Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice

Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice

DOI:
10.3791/3906-v

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10:25 min

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June 13, 2012

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, , , , , , , , ,
1Laboratory of Experimental Hematology,University of Antwerp, 2Bio Imaging Lab,University of Antwerp

Chapters

  • 00:05Title
  • 01:24Cell Preparation
  • 02:47Cell Implantation in the CNS of Mice
  • 05:14In vivo Bioluminescence Imaging and Magnetic Resonance Imaging
  • 07:44Post Mortem Histology
  • 08:45Results: Multimodal Imaging of Stem Cell Grafts
  • 09:46Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes an optimized sequence of events for multimodal imaging of cellular grafts in rodent brain using: (i) in vivo bioluminescence and magnetic resonance imaging, and (ii) post mortem histological analysis. Combining these imaging modalities on a single animal allows cellular graft evaluation with high resolution, sensitivity and specificity.

Tags

Multimodal ImagingStem Cell TransplantationCentral Nervous SystemMiceFunctional OutcomeMolecular Imaging ModalitiesStem Cell GraftingBioluminescence ImagingMagnetic Resonance ImagingHistological AnalysisCell TrackingCell SurvivalLuciferase-reporter Gene

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