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Neuroscience
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Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
 

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Article DOI: 10.3791/3907-v 09:51 min July 15th, 2012
July 15th, 2012

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Summary

We demonstrate how changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. We image intracellular calcium using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.

Tags

Intracellular Calcium Synaptic Efficacy Mollusc Aplysia Calcium Imaging Intracellular Recording Buccal Ganglion Synaptic Plasticity Homosynaptic Facilitation Calcium Orange Iontophoresis Fluorescent Dye
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