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Polymerase Chain Reaction

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Article DOI: 10.3791/3998 09:00 min
May 22nd, 2012


Summary May 22nd, 2012

PCR has emerged as a common technique in many molecular biology laboratories. Provided here is a quick guide to several conventional PCR protocols. Because each reaction is a unique experiment, optimal conditions required to generate a product vary. Understanding the variables in a reaction will greatly enhance troubleshooting efficiency, thereby increasing the chance to obtain the desired result.

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