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JoVE Journal
Biology

This content is Open Access.

Polymerase Chain Reaction
 

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Article doi: 10.3791/3998
May 22nd, 2012

Summary May 22nd, 2012

PCR has emerged as a common technique in many molecular biology laboratories. Provided here is a quick guide to several conventional PCR protocols. Because each reaction is a unique experiment, optimal conditions required to generate a product vary. Understanding the variables in a reaction will greatly enhance troubleshooting efficiency, thereby increasing the chance to obtain the desired result.

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