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Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
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Neuroscience
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JoVE Journal Neuroscience
Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

DOI:
10.3791/4008-v

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06:47 min

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April 19, 2012

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1Developmental Neurobiology Section,National Institute of Neurological Disorders and Stroke, National Institutes of Health

Chapters

  • 00:05Title
  • 01:44Pipette Preparation and Positioning
  • 02:40Dye Application
  • 04:36Incubation
  • 04:56Results: Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.

Tags

Retrograde LabelingNervesTractsSpinal RootsFluorescent DyesCalcium-sensitive DyesVoltage-sensitive DyesSolid CrystalsLocal Pressure InjectionGlass PipettesDye DiffusionPolyethylene Suction Pipette

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