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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
Journal (Immunology and Infection)
Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
Journal (Immunology and Infection)
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
 

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Article DOI: 10.3791/4040
May 21st, 2012 •
1, 1, 2, 1, 1,2
1Department of Pediatrics, Emory University, 2Department of Pathology & Laboratory Medicine, Emory University

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May 21st, 2012

Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.

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