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JoVE Journal Biology
Single Oocyte Bisulfite Mutagenesis
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Single Oocyte Bisulfite Mutagenesis

Article DOI: 10.3791/4046-v • 13:18 min • June 27th, 2012
June 27th, 2012 •
1,2,3, 3, 1,2,3
1Department of Obstretrics & Gynaecology, Schulich School of Medicine and Dentistry, University of Western Ontario, 2Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Children's Health Research Institute

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Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.

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Single Oocyte Bisulfite Mutagenesis Epigenetics Chromatin Modifications Gene Accessibility Gene Transcription DNA Methylation Repressive Mark CpG Dinucleotides Methyl Group Repressive Proteins Histone Modifications Developmental Programming Cell Differentiation Retroviral Elements X-chromosome Inactivation Genomic Imprinting Bisulfite Mutagenesis Sodium Bisulfite Deamination Uracils PCR Amplification Sequencing Conversion Events Thymines Methylated Cytosines Nucleotide Level Agarose Bead
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