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Chapters
- 00:05Title
- 00:46Cultivation and Harvest of P. putida
- 01:37Formulation Solutions, Preparing Cells for Freeze-drying, and Surface Tension Measurements
- 03:22Characterization of Formulation Freezing Behavior by Differential Scanning Calorimetry (DSC) and Freeze-drying
- 05:31X-ray Analysis of the Dry Formulations and Electron Microscopy
- 06:38Representative Freeze-drying Results
- 08:37Conclusion
Freeze-drying is often an easy and convenient way to obtain dry products of viable bacterial cells. An issue of the process is cell survival. We detail here a procedure to investigate how cell survival during freeze-drying is influenced by the properties of the formulation used.
Tags
FormulationsFreeze-dryingBacteriaCell SurvivalDehydration MethodMatrixProtectionSucroseFicoll PM400Hydroxyethyl CelluloseHydroxypropyl Methyl CelluloseP. Putida KT2440A. Chlorophenolicus A6RehydrationCharacterizingDifferential Scanning Calorimetry (DSC)Surface Tension MeasurementsX-ray AnalysisElectron MicroscopySurvival RatesPolymers
