We describe an integrated method for the precise, stereotactic implantation of human glioblastoma multiforme cells into the brains of nude mice and subsequent serial in vivo imaging to monitor growth and response to treatment of the resultant xenografts.
Baumann, B. C., Dorsey, J. F., Benci, J. L., Joh, D. Y., Kao, G. D. Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme. J. Vis. Exp. (67), e4089, doi:10.3791/4089 (2012).