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NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts
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JoVE Journal Medicine
NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

DOI:
10.3791/4115-v

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12:07 min

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July 24, 2012

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, , , ,
1Electrical and Computer Engineering Department,The George Washington University, 2Pharmacology and Physiology Department,The George Washington University

Chapters

  • 00:05Title
  • 01:25Setting Up for the Study
  • 02:44Heart Excision
  • 03:50Biventricular Cannulation
  • 07:21Signal Acquisition: Pressures, Monophasic Action Potentials, and fNADH
  • 09:08Typical Pressure, Monophasic Action Potentials, and fNADH Data
  • 11:12Conclusion

Summary

Automatic Translation

Automatic Translation

The objective is to monitor the mitochondrial redox state of isolated hearts within the context of physiologic preload and afterload pressures. A biventricular working rabbit heart model is presented. High spatiotemporal resolution fluorescence imaging of NADH is used to monitor the mitochondrial redox state of epicardial tissue.

Tags

NADH Fluorescence ImagingIsolated Perfused HeartLangendorff TechniqueCardiac PhysiologyCardiac MetabolismLV Working Heart ModelBiventricular ModelStroke VolumeCardiac OutputPressure DevelopmentMyocardial InjuryIschemiaNADH Fluorescence

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