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Chapters
- 00:05Title
- 01:21Production of Oregon Green 488-gelatin Coated Coverslips
- 04:48Plating Cells on Oregon green 488-gelatin Coated Coverslips
- 05:33Quantification of Fluorescent Gelatin Degradation by Measuring Normalized Matrix Degradation
- 09:28Results: Invadopodia-mediated ECM Proteolysis
- 13:23Conclusion
We describe the prototypical method for producing microscope coverslips coated with fluorescent gelatin for visualizing invadopodia-mediated matrix degradation. Computational techniques using available software are presented for quantifying the resultant levels of matrix proteolysis by single cells within a mixed population and for multicellular groups encompassing entire microscopic fields.
Tags
Quantitative MeasurementInvadopodia-mediatedExtracellular Matrix ProteolysisSingle And Multicellular ContextsCellular InvasionDevelopmentHomeostasisMalregulated InvasionCell MovementPathological ProcessesInflammationCardiovascular DiseaseTumor Cell MetastasisProteolytic DegradationExtracellular Matrix (ECM)Epithelial Basement MembraneEndothelial Basement MembraneVentral Actin-rich Membrane Protrusive StructuresInvadopodiaMatrix Metalloproteinases (MMPs)Tumor CellsIn Vitro AnalysisECM DegradationFluorescent MicroscopyDye-labeled Matrix ProteinsGlass CoverslipsEvaluating Matrix ProteolysisCellular Invasive Potential
