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Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
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JoVE Journal Neuroscience
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

DOI:
10.3791/4134-v

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12:48 min

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June 02, 2012

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, ,
1Department of Cell and Developmental Biology, Center for Stem Cell Biology,Vanderbilt University Medical Center, 2Department of Pharmacology, Center for Stem Cell Biology,Vanderbilt University Medical Center, 3Vanderbilt University Medical Center

Chapters

  • 00:05Title
  • 01:14Introduction
  • 01:36Prepare Plates
  • 02:18Preparation of Self Renewal and Wash Media
  • 03:06Preparation of Collagenase/Dispase
  • 04:22Isolating Vagal and Trunk Neural Tube
  • 08:21Removal of Non-Neural Ectoderm and Mesoderm
  • 11:06Removal of the Neural Tube
  • 11:46Representative Results
  • 12:35Conclusions

Summary

Automatic Translation

Automatic Translation

Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.

Tags

Neural Crest CellsEmbryonic Murine Neural TubeIsolationCultureMultipotent Progenitor PopulationEpithelial To Mesenchymal TransitionMigrationSelf-renewalAvian Neural TubeRat SystemsExplanted NC CellsProliferationTransplantationCellular PropertiesNeural Tube Explant AssayMammalian NCIsolation MethodsPost-migratory NC Progenitors

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