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Journal / Biology / Aislamiento de ratón Islet Langerhans utilizando…
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Aislamiento de ratón Islet Langerhans utilizando una combinación de colagenasa purificada y proteasa neutra

Article DOI: 10.3791/4137-v • 06:59 min • September 7th, 2012
September 7th, 2012 •
1, 2, 2, 1, 1,3,4
1Department of Pediatrics and the Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine, Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology, Indiana University School of Medicine

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Una descripción detallada de ratón aislamiento de islotes se describe utilizando la técnica de canulación en pancreático ductal in situ y la perfusión de una combinación de colagenasa purificada y proteasa neutra.

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Biología Celular número 67 Islet colagenasa el ratón la insulina la fluorescencia
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