Journal

Medicine

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

JoVE Journal
Medicine

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JoVE Journal Medicine

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

DOI:

10.3791/4162-v

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08:47 min

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August 21, 2012

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Matthias J.E. Arlt, Walter Born, Bruno Fuchs

¹Laboratory for Orthopedic Research,Balgrist University Hospital, Zurich

Chapters

00:05Title
01:12In-situ Lung Perfusion and Fixation
03:30Visualizing lacZ-tagged Metastatic Tumor Cells in Entire Lung Lobes
04:31Visualizing lacZ-tagged Metastatic Tumor Cells in Lung Cryosections
05:48Metastases Analysis in Dunn/LM8 Osteosarcoma-bearing Mice
08:00Conclusion

Summary

Automatic Translation

The novel protocol reported in the present study allows selective detection of lung metastases at single cell resolution in mice by combined in-situ lung perfusion and fixation and X-Gal staining of lacZ-tagged tumor cells.

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

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