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Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
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Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Article DOI: 10.3791/4162-v • 08:48 min • August 21st, 2012
August 21st, 2012 •
1, 1, 1
1Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich

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The novel protocol reported in the present study allows selective detection of lung metastases at single cell resolution in mice by combined in-situ lung perfusion and fixation and X-Gal staining of lacZ-tagged tumor cells.

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Improved Visualization Lung Metastases Single Cell Resolution Mice In-situ Perfusion Lung Tissue X-Gal Staining LacZ-tagged Tumor Cells Micrometastases Radiologic Imaging Therapeutic Eradication Animal Models Cancer Research Monitoring/visualization Preclinical Studies Clinical Trials Detection Techniques Micro-computer Tomography (micro-CT) Positron Emission Tomography (PET) Bioluminescence Imaging Fluorescence Imaging Optimization Techniques Primary Tumor Growth Osteosarcoma Models Ex Vivo Analysis QPCR FACS Histological Staining
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