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JoVE Journal
Chemistry

This content is Open Access.

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
 

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

Article doi: 10.3791/4189
February 4th, 2013 Usage Statistics

Summary February 4th, 2013

Ice binding proteins (IBPs), also known as antifreeze proteins, inhibit ice growth and are a promising additive for use in the cryopreservation of tissues. The main tool used to investigate IBPs is the nanoliter osmometer. We developed a home-designed cooling stage mounted on an optical microscope and controlled using a custom-built LabVIEW routine. The nanoliter osmometer described here manipulated the sample temperature in an ultra-sensitive manner.

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