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Flow Cytometry-based Purification of S. cerevisiae Zygotes
 

Flow Cytometry-based Purification of S. cerevisiae Zygotes

Article DOI: 10.3791/4197
September 21st, 2012

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Summary September 21st, 2012

To purify zygotes of S. cerevisiae, haploid cells of opposite mating type were engineered to express red or green fluorescent proteins, co-incubated to allow zygote formation, and fractionated using a flow cytometry-based protocol. The highly-enriched fraction enables subsequent "-omic" studies, recovery of initial progeny, and systematic investigation of zygote morphogenesis.

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