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Flow Cytometry-based Purification of S. cerevisiae Zygotes
 

Flow Cytometry-based Purification of S. cerevisiae Zygotes

Article doi: 10.3791/4197
September 21st, 2012 Usage Statistics

Summary September 21st, 2012

To purify zygotes of S. cerevisiae, haploid cells of opposite mating type were engineered to express red or green fluorescent proteins, co-incubated to allow zygote formation, and fractionated using a flow cytometry-based protocol. The highly-enriched fraction enables subsequent "-omic" studies, recovery of initial progeny, and systematic investigation of zygote morphogenesis.

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