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Chapters
- 00:05Title
- 01:47Extraction of Glycosphingolipids from Rat Leukemia Cells
- 02:50Separation of Neutral and Acidic Lipids
- 05:03Removal of Phospholipids from the Neutral Fraction
- 08:14Permethylation of Glycosphingolipids
- 10:04Results: Mass Spectra of Permethylated Glycosphingolipids
- 12:12Conclusion
Eine spezifische und sensitive Methode, um Einblick in die Expression von Glykosphingolipid Antigene Immunsystem Organe und Zellen zu gewinnen beschrieben. Das Verfahren nutzt die Ionenfallenmassenspektrometer ermöglicht stufenweise Fragmentierung Glycosphingolipid Moleküle zur Strukturanalyse gegenüber chemisch synthetisierten Standard.
Tags
Mass Spectrometric AnalysisGlycosphingolipid AntigensGSL’sGlycoconjugate BiomacromoleculesStructural InformationEmbryonic DevelopmentSignal TransductionImmune Receptor RecognitionSugar MoietiesIsomersLipid MoietiesFatty Acyl Chain LengthUnsaturationHydroxylationCarbohydrate PortionCeramide PortionTri-hexosylceramidesGlobotriaosylceramide (Galα4Galβ4Glcβ1Cer)Isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer)Molecular MassesSugar LinkagesBiological FunctionsModification Of Ceramide PartAlpha-galactosylceramideCytokine Secretion ProfilesInvariant NKT CellsCancer ModelsAutoimmune DiseasesStructural Analysis Of IsomersImmune Organs And Cells
