Immunology and Infection
A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
Summary October 1st, 2012
Methods for purifying the cholesterol binding toxin streptolysin O from recombinant E. coli and visualization of toxin binding to live eukaryotic cells are described. Localized delivery of toxin induces rapid and complex changes in targeted cells revealing novel aspects of toxin biology.