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Journal
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Bioengineering
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从病人的血液样本可行的循环肿瘤细胞的快速分离
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
Please note that all translations are automatically generated.
Click here for the English version.
从病人的血液样本可行的循环肿瘤细胞的快速分离
DOI:
10.3791/4248-v
•
07:32 min
•
June 15, 2012
•
Andrew D. Hughes
,
Jeff Mattison
,
John D. Powderly
3
,
Bryan T. Greene
3
,
Michael R. King
1
Department of Biomedical Engineering
,
Cornell University
,
2
BioCytics, Inc.
,
3
Carolina BioOncology Institute, PLLC
Chapters
00:05
Title
01:15
Preparation of Halloysite Nanotube Solution
01:48
Coating of Microtube Inner Surface with Halloysite Nanotubes
03:10
Preparation of Microtubes for Cell Isolation
04:05
Preparation of Samples for Cell Isolation
05:07
Cell Isolation
06:18
Results: Isolation of Circulating Tumor Cells (CTCs)
07:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
癌症患者的血液循环肿瘤细胞的分离,而造成细胞损伤。肿瘤细胞的分离,实现使用的E-选择双分子表面,除了对上皮标记的抗体。碳纳米管涂层,特别是促进癌细胞粘附造成捕获纯度高。
Tags
CTC Isolation
Circulating Tumor Cells
Viable CTC
Patient Blood Samples
Disease Progression
CTC Concentration
Personalized Therapies
Cellular Damage
Leukocytes Contamination
CTC Enumeration
Microfluidic Device
E-selectin Glycoprotein
Epithelial Markers
Nanoparticle Coating
Halloysite Nanotubes
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