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Examining BCL-2 Family Function with Large Unilamellar Vesicles
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Examining BCL-2 Family Function with Large Unilamellar Vesicles

Examining BCL-2 Family Function with Large Unilamellar Vesicles

DOI:
10.3791/4291-v

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08:35 min

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October 05, 2012

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, ,
1Department of Oncological Sciences, Department of Dermatology, The Tisch Cancer Institute, The Graduate School of Biological Sciences,Mount Sinai School of Medicine

Chapters

  • 00:05Title
  • 01:39Combine Lipids and Create a Lipid Film
  • 02:12Prepare Liposome Solution
  • 03:08Extrusion and Purification of Liposomes
  • 04:30Test Column Fractions
  • 05:33BCL-2 Family Studies Using LUVs
  • 06:11Results: BCL-2 Regulation of LUV Permeabilization
  • 07:49Conclusion

Summary

Automatic Translation

Automatic Translation

Biochemically-defined large unilamellar vesicles (LUVs) are a convenient model system to analyze BCL-2 family interactions with immediate implications in better understanding the mitochondrial pathway of apoptosis. A method to produce LUVs, along with standard BCL-2 family protein combinations and controls to examine LUV permeabilization, are presented.

Tags

BCL-2 FamilyLarge Unilamellar VesiclesApoptosisCellular StressPro-apoptotic ProteinsMitochondrial Outer Membrane Permeabilization (MOMP)Cytochrome CCaspase ActivationBH3-only ProteinsBIDBAK/BAX ActivationAnti-apoptotic BCL-2 Family ProteinsBCL-xLMCL-1BADPUMA

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