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JoVE Journal
Biology

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Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos
 

Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

Article DOI: 10.3791/4319
December 3rd, 2012

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Summary December 3rd, 2012

Described here is an in vivo technique to image sub-cellular structures in animals exposed to anoxia using a gas flow through microincubation chamber in conjunction with a spinning disc confocal microscope. This method is straightforward and flexible enough to suit a variety of experimental parameters and model systems.

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