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Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos
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Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

DOI:
10.3791/4319-v

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08:49 min

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December 03, 2012

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, ,
1Department of Biological Sciences,University of North Texas

Chapters

  • 00:05Title
  • 02:02Sample Preparation
  • 03:15Slide Preparation and Anoxia Chamber Setup
  • 05:52Microscopy in Anoxia
  • 07:36Representative Embryo Imaging Results
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

Described here is an in vivo technique to image sub-cellular structures in animals exposed to anoxia using a gas flow through microincubation chamber in conjunction with a spinning disc confocal microscope. This method is straightforward and flexible enough to suit a variety of experimental parameters and model systems.

Tags

Time Lapse MicroscopyAnoxia-induced Suspended AnimationC. Elegans EmbryosStress ResistanceGenetic MutantsTransgenic StrainsFusion ProteinsFluorescently Labeled Reporter ProteinsMitosisIn Vivo ImagingSub-cellular StructuresAnoxic StressGas Flow SetupHigh-powered MicroscopeMicroincubation ChamberNitrogen Gas Flow ThroughSpinning Disc Confocal MicroscopeControlled EnvironmentGFP-tagged Gamma TubulinHistoneCell Division ArrestOxygen Deprivation

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