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Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
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Chapters
- 00:05Title
- 01:53Preparation of the Mouse Lung
- 04:02Enzymatic Digestion of the Lung Tissue
- 05:40Preparation of the Lung Cell Suspension
- 08:02Antibody Staining for Flow Cytometric Cell-sorting
- 08:58Cell-sorting
- 10:36Representative Results
- 14:07Conclusion
We describe the rapid isolation of primary murine type II alveolar epithelial cells (AECII) by flow cytometric negative selection. These AECII show high viability and purity and are suitable for a wide range of functional and molecular studies regarding their role in respiratory conditions such as autoimmune or infectious diseases.
Tags
Flow Cytometric IsolationPrimary Murine Type II Alveolar Epithelial CellsFunctional StudiesMolecular StudiesImmune RegulationCytokine ProductionAntigen-presenting CellsInfectionT-cell Mediated AutoimmunityClinical ContextsAirway Hyper-reactivityInfectionsCell LinesLarge Numbers Of CellsComplex ProcessesAutoimmune Inflammation
