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Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells

DOI:
10.3791/4333-v

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13:18 min

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December 29, 2012

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, ,
1Department of Microbiology and Immunology,University of Maryland

Chapters

  • 00:05Title
  • 01:36Generation of Artificial Antigen Presenting Cells (aAPC)
  • 03:39Isolation of CD161+CD3+ Cells
  • 07:22aAPC-mediated NKT Cell Expansion
  • 08:16Functional Test: aAPC-mediated Stimulation of NKT Cells
  • 08:47Results: aAPC-mediated Activation and Expansion of NKT Cells
  • 12:48Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a method for activating and expanding human NKT cells from bulk T cell populations using artificial antigen presenting cells (aAPC). The use of CD1d-based aAPC provides a standardized method for generating high numbers of functional NKT cells.

Tags

Artificial Antigen Presenting Cell (aAPC)Natural Killer T (NKT) CellsLipid AntigenCD1 MoleculesInvariant TCRα ChainCanonical NKT (iNKT) CellsCD4-CD8- Thymic Precursor T Cellsα-Galactosylceramide (α-GalCer)CytokinesAdoptive ImmunotherapyAutologous Antigen Presenting CellsMonocyte-derived DCCostimulatory Molecules

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