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Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

JoVE Journal
Biology

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JoVE Journal Biology

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

DOI:

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10:09 min

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February 28, 2013

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Wei-Meng Woo*¹, Scott X. Atwood*¹, Hanson H. Zhen, Anthony E. Oro

¹Program in Epithelial Biology,Stanford University School of Medicine

Chapters

00:05Title
01:31Isolating and Preparing Mouse Skins
03:17Isolating Primary Dermal or Epidermal Cells from Skins
05:07Grafting Primary Dermal and Epidermal Cells
07:57Histological Analysis of Hair Follicle Growth
09:32Conclusion

Summary

Automatic Translation

Tissue-specific analysis of a hair follicle regeneration model using lentivirus to mediate gain- or loss-of-function.

Tags

Genetic Analysis Epithelial-mesenchymal Signaling Hair Regeneration Hair Follicle Morphogenesis Organ Development Lentivirus Overexpression ShRNA-mediated Knockdown Gain-of-function Analysis Loss-of-function Analysis Primary Mouse Keratinocytes Dermal Cells Dermal Papilla Cells Hair Follicles Nude Mice Genetic Models

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