A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:20Labeling of Single Cells
- 02:58Embryo Laser Wounding, Recovery, and Analysis
- 04:29Representative Muscle Wounding Results
- 06:40Conclusion
The method presented here comprises the precise injury of live zebrafish embryos with high-energy laser pulses and the subsequent analysis of these injuries and their recovery with time. We also show how genetically labeled single or groups of skeletal muscle cells can be tracked during and after laser light induced damage.
Tags
ZebrafishEmbryonic Skeletal MuscleLaser-inflicted InjuryMuscle RegenerationMyotoxin InjectionsMuscle TransplantationsDenervation-devascularization Induced RegenerationIntensive ExerciseMurine Muscular Dystrophy ModelsMdx MouseRunzel2Sapje3Antisense Oligonucleotide MorpholinosDystrophy-associated GenesGalanthamineAcetylcholinesterase InhibitorHypercontractionLaser-mediated Cell AblationTwo-photon Laser
