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Neuroscience

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Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
 

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

Article doi: 10.3791/4377
December 23rd, 2012

Summary December 23rd, 2012

Xenopus laevis provides an ideal model system for studying cell fate specification and physiological function of individual retinal cells in primary cell culture. Here we present a technique for dissecting retinal tissues and generating primary cell cultures that are imaged for calcium activity and analyzed by in situ hybridization.

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