Xenopus laevis provides an ideal model system for studying cell fate specification and physiological function of individual retinal cells in primary cell culture. Here we present a technique for dissecting retinal tissues and generating primary cell cultures that are imaged for calcium activity and analyzed by in situ hybridization.

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

•

•

•

Chapters

Summary

Automatic Translation

Tags

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium

Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons

Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

Neural Explant Cultures from Xenopus laevis

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

A Simple Behavioral Assay for Testing Visual Function in Xenopus laevis

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Read Article