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JoVE Journal Neuroscience
Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
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Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

Article DOI: 10.3791/4377 • 10:22 min • December 23rd, 2012
December 23rd, 2012 •
*1, *1, 1, 1, 1, 1
1Department of Biology, College of William and Mary
* These authors contributed equally

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Summary
Automatic Translation

Xenopus laevis provides an ideal model system for studying cell fate specification and physiological function of individual retinal cells in primary cell culture. Here we present a technique for dissecting retinal tissues and generating primary cell cultures that are imaged for calcium activity and analyzed by in situ hybridization.

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Dissection Culture Analysis Xenopus Laevis Embryonic Retinal Tissue Neural Plate Vertebrate Retina Clinical Research Basic Research Retinal Disease Neuronal Cell Type Determination Differentiation Ganglion Cells Amacrine Cells Horizontal Cells Photoreceptors Bipolar Cells Müller Glial Cells Layered Structure Primary Cell Culture Presumptive Retinal Cells Developmental Stages Cell Fate Specification Interactions With Neighboring Tissues
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