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Dissektion, Kultur, og analyse af<em> Xenopus laevis</em> Embryonic retinavæv
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JoVE Journal Neuroscience
Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

Dissektion, Kultur, og analyse af<em> Xenopus laevis</em> Embryonic retinavæv

DOI:
10.3791/4377-v

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10:22 min

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December 23, 2012

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, , , , ,
1Department of Biology,College of William and Mary

Chapters

  • 00:05Title
  • 01:26Dissection
  • 06:41Dissociation of Tissue and Plating of Cells
  • 08:24Results: Analysis of Xenopus laevis Embryonic Retinal Tissue
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

Xenopus laevis er et ideelt modelsystem til at studere celle-skæbne specifikation og fysiologiske funktion af individuelle retinale celler i primær cellekultur. Her præsenteres en teknik til dissekering retinale væv og frembringelse af primære cellekulturer, der er afbildet for calcium-aktivitet og analyseret ved in situ hybridisering.

Tags

DissectionCultureAnalysisXenopus LaevisNeural PlateVertebrate RetinaClinical ResearchBasic ResearchRetinal DiseaseNeuronal Cell Type DeterminationDifferentiationGanglion CellsAmacrine CellsHorizontal CellsPhotoreceptorsBipolar CellsMüller Glial CellsLayered StructurePrimary Cell CulturePresumptive Retinal CellsDevelopmental StagesCell Fate Specification

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