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Chapters
- 00:05Title
- 01:27Preparation of cDNA Expression Library
- 05:43Cell Seeding and Transfection
- 07:10Image Acquisition
- 08:51Results: Changes in Grb2 Localization by cDNA Expression
- 10:24Conclusion
A high-content screening method for the identification of novel signaling competent transmembrane receptors is described. This method is amenable to large-scale automation and allows predictions about in vivo protein binding and the sub-cellular localization of protein complexes in mammalian cells.
Tags
High-content ImagingGrb2 Signaling ComplexesExpression CloningSignal TransductionGrowth Factor ReceptorsDownstream Signaling CascadesMitogenic SignalingSH2 DomainSH3 DomainGTPase Son-Of-Sevenless (SOS)Phosphorylated Tyrosine ResiduesSOS-Ras-MAP Kinase Signaling CascadePositive And Negative Regulator Of SignalingReceptor EndocytosisMicroscopy AssayPlasma Membrane ReceptorsEpidermal Growth Factor Receptor (EGFR)GFP-Grb2
