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JoVE Journal
Biology

This content is Open Access.

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
 

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

Article DOI: 10.3791/4382
October 30th, 2012

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Summary October 30th, 2012

A high-content screening method for the identification of novel signaling competent transmembrane receptors is described. This method is amenable to large-scale automation and allows predictions about in vivo protein binding and the sub-cellular localization of protein complexes in mammalian cells.

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