A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
Summary October 16th, 2012
A method by which gene expression in the neural tube can be downregulated in a cell type-specific, traceable manner is described. We demonstrate how in ovo electroporation of microRNA-based plasmids that elicit spatiotemporally controlled RNA interference can be used to investigate commissural axon guidance in the developing neural tube.