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Neuroscience

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JoVE Journal Neuroscience
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In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations

Article DOI: 10.3791/4384
October 16th, 2012 •
1, 1
1Institute of Molecular Life Sciences, University of Zurich

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October 16th, 2012

A method by which gene expression in the neural tube can be downregulated in a cell type-specific, traceable manner is described. We demonstrate how in ovo electroporation of microRNA-based plasmids that elicit spatiotemporally controlled RNA interference can be used to investigate commissural axon guidance in the developing neural tube.

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