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Journal
/
Biology
/
Freezing Human ES Cells
JoVE Journal
Biology
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JoVE Journal
Biology
Freezing Human ES Cells
Freezing Human ES Cells
DOI:
10.3791/50-v
•
08:00 min
•
October 12, 2006
•
Erin Trish
,
John Dimos
,
Kevin Eggan
1
Department of Molecular and Cell Biology
,
Harvard
Chapters
00:20
Preparation
00:55
Trypsinize cells
05:09
Resuspend cells and store in cryogen tubes
06:29
Add freezing media
06:53
Transfer cells to freezer
Summary
Automatic Translation
English (Original)
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Automatic Translation
Here we demonstrate how our lab freezes HuES human embryonic stem cell lines.
Tags
Freeze
Human ES Cells
Lab
HuES Human Embryonic Stem Cell Lines
Culture
PBS
Residual Media
Trypsin Reaction
Incubate
Rounding
Lifting Off
Pipetting
Conical Tube
HES Cell Media
Spin
Resuspend
Concentration
Freezing Media
Freezing Vials
-80C
Liquid Nitrogen
Long Term Storage
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