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JoVE Journal Neuroscience
Undersøqelse DNA-skader i hippocampus neuroner Brug af Comet Assay
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Undersøqelse DNA-skader i hippocampus neuroner Brug af Comet Assay

Article DOI: 10.3791/50049 • 10:00 min • December 19th, 2012
December 19th, 2012 •
1, 2, 1,3
1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

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Det comet assay er en effektiv måde til afsløring af enkelt-og dobbelt-streng pauser, herunder alkali-labile sites og DNA-DNA/DNA-protein tværbindinger på DNA i alle celler, herunder hippocampus neuroner. Fremgangsmåden drager fordel af den differentielle vandring af DNA i et elektrisk felt på grund af forskelle i mængden af ​​DNA-beskadigelse.

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Neuroscience genetik cellebiologi Medicin Cancer Biology anatomi fysiologi DNA DNA-skader dobbeltstreng pause enkeltstrenget pause reparation neuroner comet assay cellekultur
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