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Journal / Neuroscience / Comet Assay kullanma Hipokampal Nöronlar DNA…
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JoVE Journal Neuroscience
Comet Assay kullanma Hipokampal Nöronlar DNA Hasarı assaying
Horizontal Hippocampal Slices of the Mouse Brain
Journal (Neuroscience)
Horizontal Hippocampal Slices of the Mouse Brain
Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
Journal (Neuroscience)
Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
 
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Comet Assay kullanma Hipokampal Nöronlar DNA Hasarı assaying

Article DOI: 10.3791/50049 • 10:00 min • December 19th, 2012
December 19th, 2012 •
1, 2, 1,3
1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

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Comet assay alkali labil siteleri ve hipokampal nöronlar da dahil olmak üzere tüm hücrelerde DNA üzerinde DNA-DNA/DNA-protein çapraz bağlantılar dahil tek ve çift zincir kırıkları, tespit etmenin en etkin yoludur. Yöntem DNA hasar miktarının farklılıklara bağlı olarak, bir elektrik alanı içinde DNA diferansiyel göç yararlanır.

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Nörobilim Sayı 70 Genetik Hücresel Biyoloji Tıp Kanser Biyolojisi Anatomi Fizyoloji DNA DNA hasarı çift iplikli sonu tek iplikli mola onarım nöronlar comet assay hücre kültürü
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