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JoVE Journal
Immunology and Infection

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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
 

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Article DOI: 10.3791/50058
February 2nd, 2013

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Summary February 2nd, 2013

We describe a single-cell high-throughput assay to measure cytotoxicity of T cells when incubated with tumor target cells. This method employs a dense, elastomeric array of sub-nanoliter wells (~100,000 wells/array) to spatially confine the T cells and target cells at defined ratios and is coupled to fluorescence microscopy to monitor effector-target conjugation and subsequent apoptosis.

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