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JoVE Journal
Biology

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Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
 

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

Article doi: 10.3791/50074
March 22nd, 2013 Usage Statistics

Summary March 22nd, 2013

Isolation and culture of myofibers is the gold standard in vitro system to study the transition of satellite cells through quiescence, activation and differentiation. Importantly, the single myofiber culture system preserves the myofiber/stem cell association, which is an essential component of the muscle stem cell niche.

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